By Sidney Fleischer and Lester Packer (Eds.)

ISBN-10: 0121819523

ISBN-13: 9780121819521

The seriously acclaimed laboratory commonplace, Methods in Enzymology, is among the such a lot hugely revered courses within the box of biochemistry. when you consider that 1955, every one quantity has been eagerly awaited, usually consulted, and praised by way of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct this day - actually an important e-book for researchers in all fields of existence sciences

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Extra resources for Biomembranes - Part C: Biological Oxidations

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69, 169 (1971). ~a M. J. Coon, T. A. Van der Hoeven, S. B. Dahl, and D. A. Haugen, this volume [10]; and D. Ryan, A. Y. H. Lu, and W. Levin, this volume [11]. 2o H. Remmer, H. Greim, J. B. Schenkman, and R. W. Estabrook, this series, Vol. 10, p. 703. [2] M1CROSOMAL ELECTRON-TRANSPORT REACTIONS 47 4. The further reduction of the ternary complex of oxygen, substrate, and cytochrome P-450 by an electron donated from the reduced flavoprotein (fpl) or (possibly) reduced cytochrome b5 5. Activation of oxygen for interaction with the organic substrate 6.

Biol. Chem. 244, 6134 (1969). [3] USE OF PERFUSION OF LIVER AND OTHER ORGANS 59 are more or less elaborate methods of perfusion, which are currently being further developed. The isolated perfused lung has been used in a number of studies of fatty acid metabolism and also of the metabolism of xenobiotics, particularly environmental pollutants to which the lung is exposed. The rabbit lung is perfused either with a mixture of rabbit plasma and saline ~4 or with heparinized autologous whole blood 5s in a recirculating system of chemically inert surfaces.

1). Peristaltic pump, oxygenator, and thermostat can be obtained commercially (see Ross 4) or constructed from simple parts. A pump may be built from a motor 17 and polypropylene rollers acting on silicone 11 T. Biicher and H. Sies, in "The Use of Isolated Liver Cells and Kidney Tubules in Metabolic Studies" (J. M. Tager, J. R. Williamson, and H. D. ), p. 41. , Amsterdam, 1976. 12 H. Schimassek, Biochem. Z. 336, 460 (1%3). ~a R. Hems, B. D. Ross, M. N. Berry, and H. A. Krebs, Biochem. J. 101,284 (1966).

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Biomembranes - Part C: Biological Oxidations by Sidney Fleischer and Lester Packer (Eds.)

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