By Amélie Garénaux; et al

ISBN-10: 1616681098

ISBN-13: 9781616681098

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Sample text

Coli [413]. In E. coli, this GASP phenotype is related to mutations in the rpoS or lrp gene resulting in attenuated RpoS or inactive LRP, and this increased fitness is dependent on the pH and nutrient environment [107, 417]. In C. jejuni, Martinez-Rodriguez et al. [239] were unable to clearly demonstrate the conventional GASP phenotype as described in E. coli. Nevertheless, the phenotypic changes observed in C. jejuni appeared to be stable because they were maintained in subculture suggesting the emergence of a new strain with advantageous properties in terms of survival in starvation.

Viable but Not Cultivable Cells of Campylobacter jejuni The concept of viable but not cultivable cells (VBNC) was developed after observations of variations between bacterial counts on classical media and those using microscopic methods. The difficulty is to know exactly in which physiological state the non cultivable bacteria are: lethally injured, in a degenerescent way, or in a strategically survival state. In 1987 Roszak and Colwell [323] proposed the VBNC concept to describe non cultivable bacteria conserving metabolic activities.

In addition, in January 2007, one can identify at least 13 sequencing projects of Campylobacter, a very great majority realized by the TIGR (Table 7B) and concerning several Campylobacter species (viz. C. lari, C. coli, C. upsaliensis and C. fetus) and strains. Most of these sequences are now at the assembly stage and sequence information will be soon available in the NCBI database. The availability of these sequences opens new perspectives for comparative genomic studies on epsilon-proteobacteria and possibly, will lead to the discovery of novel molecular characteristics useful for diagnostics.

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Better understanding the Campylobacter conundrum by Amélie Garénaux; et al

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