By Lemuel Wingard (Eds.)
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Additional resources for Applied Biochemistry and Bioengineering. Enzyme Technology
Spray-drying has some other disadvantages: the process is expensive (about 5-100/liter of water removed), the enzymes are subjected to fairly high tempera tures and oxidizing conditions, impurities are not removed in the pro cess, and finally the product has a low weight/volume ratio. An advan tage is that spray-dried preparations usually are easily soluble. Precipitation is preferred when some purification is desired in the process. T w o methods are used by the industry: solvent precipitation and salt precipitation.
T h e liquid must b e kept at a low tem perature ( 3 0 ° - 5 0 ° C ) . Nevertheless it is difficult to prevent activity losses o f — 1 0 % even for very stable enzymes. An additional disadvan tage o f this method is that the low temperature makes microbial growth possible, so that frequent cleaning o f equipment and careful control o f the product are necessary. Another problem is that the drysubstance content o f the broth increases with evaporation, in some cases causing high viscosity and difficult handling.
And Lee, Β. K. (1975). , 15. , and Quinto, C. M. (1975). Appl. Microbiol. 30, 750. Sargeant, K. (1974). In "Industrial Aspects of Biochemistry" (B. ), pp. 3-22. North-Holland, Amsterdam. Savidge, Τ. , and Cole, M. (1975). In "Methods in Enzymology" (J. H. ), Vol. XLIII, pp. 705-721. Academic Press, New York. Sk0t, G. (1978). In "Enzyme Engineering" (G. B. Broun, G. Manecke, and L. B. ), Vol. 4, pp. 33-39. Plenum, New York. Solomons, G. L. (1977). In "Biotechnological Applications of Proteins and Enzymes" (Z.
Applied Biochemistry and Bioengineering. Enzyme Technology by Lemuel Wingard (Eds.)